The Applied Genetics Group at the National Institute of Standards and Technology (NIST) has been involved in the testing of integrated rapid DNA instruments since the prototype stage in the fall of 2012 and began robustly testing the maturity of these rapid DNA instruments in 2013. The three maturity assessments conducted by NIST focused on the genotyping success for the current CODIS core STR markers (at the time of each assessment), peak height ratio balance, base pair sizing precision, and stutter artifacts for each of the instruments and corresponding STR kit chemistries. Success was measured by complete and concordant genotyping when compared to truth data provided by traditional laboratory analysis.
Rapid DNA Maturity Assessment
(July-September 2018)
The goal of this maturity assessment was to measure the status of the rapid DNA typing technology for the 20 CODIS core loci in support of rapid DNA implementation within the booking station environment. Nine laboratories, which spanned across U.S. federal and state laboratories, police agencies, and commercial vendors, tested a total of 240 samples (20 per instrument across 12 instruments) with either the FlexPlex chemistry or Globalfiler Express.
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Rapid DNA Maturity Assessment
(October-December 2014)
The goal of this maturity assessment was to measure the status of the rapid DNA typing technology for the 13 CODIS core loci in support of accredited laboratory use and future external implementation. Seven laboratories, which spanned across U.S. Federal, State, and private laboratories tested a total of 280 samples (20 per instrument across 11 instruments) with either the PowerPlex 16 chemistry or Globalfiler Express.
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Rapid DNA Interlaboratory Study
(July-August 2013)
The goal of the interlaboratory study was to provide a snapshot in time of the state of rapid DNA typing. This study was not a comparison of developers. Three federal laboratories participated in this interlaboratory study testing a total of 350 samples (50 per instrument across 7 instruments) with the PowerPlex 16 chemistry.
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